International Journal of Pathogen Research

Nipah virus (NiV) is a high-consequence zoonotic pathogen associated with severe human disease and substantial outbreak risk. Although no sustained transmission has been reported in the Philippines since the 2014 outbreak involving 17 human cases and 9 deaths, ecological evidence of viral circulation in bat reservoirs and increasing regional outbreaks emphasize the need for proactive national preparedness. This study conducted a systematic review and national gap analysis to assess Nipah virus readiness in the Philippines, focusing on laboratory diagnostic capacity, biosafety and biosecurity systems, and specimen referral and transport networks. A comprehensive search of peer-reviewed literature and authoritative operational sources published between 2000 and 2025 was performed following PRISMA 2020 guidelines. Benchmarks included published WHO guidance on diagnostic preparedness, integrated laboratory networks, genomic surveillance, and HCID readiness expectations. Data were synthesized thematically and compared against international preparedness benchmarks for high-consequence infectious diseases. Preparedness was classified qualitatively through benchmark-referenced comparison across the three domains (diagnostics, biosafety/biosecurity, and specimen referral), without numerical scoring. ‘Intermediate preparedness’ denotes the presence of functional reference-level capacity and documented capacity-building initiatives, alongside persistent structural and operational gaps that limit decentralization, integration, and rapid turnaround at subnational levels. Twenty-one sources met inclusion criteria, comprising academic studies and operational reports. The findings indicate that the Philippines has achieved intermediate preparedness, characterized by strengthened surveillance, reference-level molecular diagnostic capability, and coordinated border health measures. Key gaps include centralized confirmatory testing, uneven subnational access to molecular diagnostics, limited public documentation of biosafety governance, and persistent inter-island referral and cold-chain constraints, uneven subnational access to diagnostics at subnational levels, limited public documentation of biosafety governance, and logistical challenges in specimen referral across the archipelago. While sector-specific surveillance systems have been strengthened, integration of human, animal, and environmental surveillance data under a unified One Health framework remains limited. In the Philippine context, relevant One Health touchpoints include bat and wildlife surveillance activities, veterinary reporting channels for unusual animal morbidity/mortality, and national inter-agency coordination mechanisms; however, routine, bidirectional integration of animal and environmental signals into human diagnostic escalation and outbreak response workflows is not consistently described in publicly available operational documentation. Strengthening decentralized diagnostics, reinforcing biosafety systems, and optimizing specimen referral pathways are essential to improve early detection and rapid containment. Addressing these gaps would shorten time-to-confirmation, strengthen healthcare and laboratory risk controls, and improve early containment capacity for suspected Nipah events in an archipelagic setting.