New Protein Settings to Support in vivo Antimalarial Activity in Plasmodium berghei Infected Mice after Garlic-Arteether Therapy
Vathsala Palakkod Govindan *
Undergraduate Programme, Indian Institute of Science, Bengaluru, India.
P. Krishna Murthy
Department of Biochemistry, Bharathidasan University, Tiruchirapalli, TN, India.
*Author to whom correspondence should be addressed.
Abstract
Many malaria endemic nations are pursuing malaria elimination and these technical challenges require the development of integrated approaches, among which safe and effective malaria vaccines could be a crucial tool. Due to non-availability of malaria vaccine, the control efforts rely heavily on treatment with new antimalarial agents preferably acting on newer targets. In this study, the protected serum proteomics after garlic and arteether combination treatment of P.berghei infected mice has been analyzed by western blotting. One of the identified host parasites specific proteins, peptidyl-prolyl-cis-trans isomerase A (PPIA) is known to catalyze the interconversion of the cis and trans and mediate certain protein folding events both in in vitro and in vivo conditions. This study hypothesizes that, overexpressed PPIA might lead to misfold of the parasite protein which are needed for parasite multiplication and in turn lead to the parasite death or in the protection of combination drug treated samples.
Keywords: Plasmodium berghei, Malaria, Serum protein, peptidyl-prolyl-cis-trans isomerase A, over expression, Misfolding, Protection