Microbial Contamination Potential and Antibiotic Resistance Profile of Staphylococcus aureus and Escherichia coli Isolated from Ipomoea batatas Leaf
Paul Yao Attien *
Laboratory of Biochemistry and Microbiology (LBM), UFR Agroforestry, University of Jean Lorougnon Guédé of Daloa, BP 150 Daloa, Côte d’Ivoire.
Clément Kouassi Kouassi
Laboratory of Biochemistry and Microbiology (LBM), UFR Agroforestry, University of Jean Lorougnon Guédé of Daloa, BP 150 Daloa, Côte d’Ivoire. and Department of Food Science and Technology, Laboratory of Food Biotechnology and Microbiology, University of Nangui Abrogoua, Abidjan, 02 BP 801 Abidjan 02, Côte d’Ivoire.
Mamadou Wele
Laboratory of Integrative and Exploratory Tropical Biology, Faculty of Science and Technology, University of Science, Technology and Technology of Bamako, Mali.
Haziz Sina *
Laboratory Biology and Typing Molecular in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
Lamine Baba- Moussa
Laboratory Biology and Typing Molecular in Microbiology, Faculty of Science and Technology, University of Abomey-Calavi, 05 BP 1604 Cotonou, Benin.
*Author to whom correspondence should be addressed.
Abstract
Traditional leafy vegetables are recommended because there are important sources of nutrients and they protect against certain non-communicable diseases. This study aimed at assessing the microbiological quality of Ipomoea batatas leaves and evaluating the susceptibility to antibiotics of isolated S. aureus and E. coli strains.
Heighten samples of I. batatas leaves collected were analyzed for research / enumeration of mesophilic total aerobic flora, enterobacteriaceae, yeasts, molds, fecal coliforms, fecal streptococci, Staphylococcus aureus, Escherichia coli and Salmonella strains and Escherichia coli, Staphylococcus aureus. The microorganisms were identified using appropriate medium and classical biochemical tests. The susceptibility to eleven conventional antibiotics was investigated by the disk diffusion method.
The I. batatas leaves showed high contamination by various flora, in particular flora indicative of general pollution (aerobic mesophilic germs, yeasts and molds, enterobacteria) and flora of fecal origin (fecal coliforms and fecal streptococcus). In addition, the presence of pathogenic species such as Staphylococcus aureus, Escherichia coli and Salmonella sp. was noted in the samples analyzed. Globally, the highest resistance proportion has been observed with penicillin (~65%) and none of the S. aureus strains was resistant to vancomycin.
From a microbiological point of view, Ipomoea batatas leaf intended for consumption is not suitable for human consumption. It is therefore important to educate producers to improve their production route in order to have better microbiological quality I. batatas leaves.
Keywords: Microbial contamination, Ipomoea batatas leaf, pathogenic bacteria, Ivory Coast.